About Tobramycin Aminoglycoside derivative, Antibiotic. Mechanism of Action of Tobramycin Tobramycin exerts its bactericidal action against gram- negative organisms & some of gram positive organisms by inhibiting bacterial protein synthesis. The process involves 1. Penetration of bacterial cell membrane & binding directly to the bacterial ribosome, 2. Misleading of m-RNA codon by bacterial ribosome, 3. Formation of peptide chains with wrong amino acid sequence which gets incorporated into bacterial cell membrane and alters its permeability, 4. Bacterial cell lysis. Some bacterial strains like pseudomonas are more sensitive to Tobramycin than Gentamicin. Indications for Tobramycin 1. Infections caused by pseudomonas, proteous, staphylococcus, klebsiella, E coli, serratia, enterobacter etc 2. Cystic fibrosis with pseudomonas infections: 3. Ocular infections 4. Gastro intestinal diseases 5. Urinary tract infections 6. Endocarditis 7. Meningitis