About Chloramphenicol Bacteriostatic antibiotic, A systemic broad ? spectrum antibacterial agent. Mechanism of Action of Chloramphenicol Chloramphenicol is a broad spectrum antibiotic which is primarily bacteriostatic & exerts some bactericidal action at higher concentration. It acts by inhibiting bacterial protein synthesis. Chloramphenicol attaches to 50S subunit of ribosome and interfering with transfer of elongating peptide chain to the newly attached aminoacyl-tRNA at the ribosome-m RNA complex. It prevents the access of aminoacyl-tRNA to the acceptor site for amino acid incorporation. By acting as a peptide analogue it inhibits peptide bond formation