Flourescein eye ampoule is used during medical examinations to identify problems with the surface of the eye such as scratches, ulcers, or foreign bodies. It contains a special dye that reacts with blue light, clearly showing the affected areas, which helps the doctor in diagnosing the condition accurately. It is also used to detect corneal disorders and evaluate the efficiency and flow of tears. It can also be used to measure eye pressure when used with certain devices. The ampoule must be used under direct medical supervision, taking care not to touch the tip of the ampoule to the eye and to maintain its sterility throughout the period of use. It is preferable to store it in a cool place away from light. Therefore, Flourescein is one of the important and accurate means that help in diagnosing many eye diseases.